This manuscript is focused on the matter concerning the emissions of the greenhouse gases produced by the road freight transport mode. Those emissions influence the ozone layer structure and help to create the greenhouse impact causing the global warming, i.e. issues particularly related to the weather change aspects as well as extreme weather occurrences. In the first part of the paper, the calculation approaches, energy consumption examination as well as evaluation of emissions of the greenhouse gases created by the transport modes operation are performed by the EN 16258:2013 standard. The research chapter outlines the implementation of the approach technique to particular consignment on a specific transport section, while the sum of the energy consumption and greenhouse gases emissions production per pallet of goods weighing 1000 kg is specified. The resulting values form the fundamentals for declarations of energy consumption and greenhouse gas emissions. The proper and precise examinations of energy consumption and emissions production can help to identify the share of external costs for specific transport modes quite accurately. This ensures that such costs are fairly carried by the individual producers thereof.