This contribution describes the subjective assessment process of indoor environmental conditions in university classrooms. The university campus consisting of several buildings is situated on the outskirts of the town of České Budějovice (South Bohemia, Czech Republic). A high level of indoor environment quality (IEQ) is a key factor to achieve healthy environments in buildings. Suitable indoor air quality is more important in schools and universities (no-industrial civic amenities) than in many other buildings. Indoor air quality in the university environment affects learning processes, concentration, and productivity of students. It also could affect the health of students and teachers in long- term. Students are at greater risk because of the hours spent in educational facilities. The evaluation of the subjective assessment of the quality of the indoor environment at university classrooms is described within the paper.