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Underpasses play an important role in ensuring permeability of line traffic structures (motorways, expressways) for feral species. There are many studies, articles and methodological guidebooks available describing proper design principles for technical parameters of underpasses and their placement within the terrain. Width, height and length are the main technical parameters affecting wildlife migration. Wildlife behaviour and their requirements for the mentioned technical parameters of underpasses also have been examined in depth. In practically all cases, underpass functionality grows with increasing width and height. Of course, this also results in greater construction costs. The objective should therefore be to find a balanced compromise enabling “sufficient” functionality while maintaining “reasonable” costs. Data envelopment analysis (DEA) provides a way to identify “good” solutions in the sense of “sufficient” underpass functionality with “reasonable” total costs. Underpass functionality calculations were based on a methodology for calculating migration potentials (Žák and Florian, 2013). Total costs were established according to actual construction work prices in the Czech Republic, including prices for preparations, design, construction, maintenance, and demolition. The results indicate that DEA can be used to find “good” solutions and can be of assistance in particular when planning measures to ensure motorways are permeable to wildlife.