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Today´s world is reflected in words such as globalization, economic crisis, market economy and competitiveness. All these influence business entities, some positively and others negatively. An enterprise that does not keep pace with current trends, innovates, seek improvements in quality or assess the effectiveness of its activities, is at risk of stagnating. Enterprises have begun to realize that one of the key factors of their success is their employees. If employees are satisfied, they are able to influence the effectiveness, productivity and the overall performance of the enterprise. Similar trends also prevail in logistics enterprises in Slovakia. The aim of this study was to analyse organizational culture in logistics enterprises because it is one of the key factors affecting the quality of the products and the overall competitiveness of an enterprise. Utilizing the Organizational Culture Assessment Instrument (OCAI) we diagnosed the dominant trend within Slovak logistics enterprises. On the whole these enterprises have a hierarchical organizational culture. However, employees are of the opinion that there should be a more familial work environment, typical of a clan organizational culture. Whilst enterprises continue to underestimate this factor, they will miss an important tool with which to lead their employees more effectively and achieve better success rates in a tough competitive environment.