The chapter “South Bohemian Region and Russia” of the expert collective monograph “South Bohemian Region in Global Economy” deals with the level and forms of cooperation of South Bohemian organizations with the Russian Federation. Nearly 100 South Bohemian organizations from different fields were approached, of which 21 filled in a detailed questionnaire. The questionnaires were evaluated by means of a method of a quantitative and qualitative research for collecting and interpreting primary information and data. We found out that the most of the contacted organizations enjoy pleasant experience with Russian partners and plan on extending their activities in the Russian Federation in spite of substantially worsened conditions on the Russian market. As a matter of fact, IMG Bohemia, Fruko-Schulz and Budějovický Budvar etc. rank among the most active organizations. The South Bohemian organizations cooperate mostly with partners from the European (western) part of Russia (with the exception of Sverdlovskaya oblast closely to the Urals) under the leadership of Moscow and Yekaterinburg and also often with the other countries of the former Soviet Union. The South Bohemian Region lacks a specialized institution, e.g. South Bohemian cluster to encourage the cooperation with the Eastern European regions.