The chapter “Analysis of Innovation Activieties of Enterprises” of the expert collective monograph “The South Bohemian Region in Global Economy” showed that enterprises in the South Bohemian Region do not differ from innovators in other regions. The key problem is still the transition from expenditures on scientific and research innovation to innovation outputs in the form of new products and services. We found out that companies that gain information from their parent company and follow feedback and information from their clients invest more funds in innovation projects. The tested hypotheses can be evaluated as follows: 1) the South Bohemian Region is not one of the regions where enterprises decide on innovations more often. There are no major differences between all of the regions of the Czech Republic. 2) Sources of information influence innovation expenditures. 3) Innovation strategies (market expansion, higher flexibility etc.) have no statistical influence. Cooperation and gaining data on new technologies are shown to be the most important.